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mirna 3´utr target expression clone for human myb  (Genecopoeia)


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    Structured Review

    Genecopoeia mirna 3´utr target expression clone for human myb
    Mirna 3´Utr Target Expression Clone For Human Myb, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 93/100, based on 244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/utr+target+expression/miRNA+3%C2%B4UTR+target+expression+clone+for+Human+MYB/custom%40hmit157288-mt05%4042048347
    Average 93 stars, based on 244 article reviews
    mirna 3´utr target expression clone for human myb - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Reporter Assay:

    Article Title: MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells
    Article Snippet: The FOXO1-related fluorescence was firstly evaluated in EC transfected with fluorescent pEGFP rbFOX1 plasmid (63085, Addgene, Watertown, MA, USA) and Dreamfect 1000 reagent (DF41000, OZ Biosciences, Quimigen, Madrid, Spain), to assess the best transfectant experimental conditions. .. Dual-luciferase reporter assay was then performed by Firefly & Renilla luciferase single tube assay kit (30081-T, Biotium, Fremont, CA, USA), following manufacturer's protocols, on EC co-transfected with customized luciferase plasmid containing the 350–550 region of the 3′UTR target expression clone for human FOXO1 (CS-HmiT127365-MT06-01, GeneCopoeia, Rockville, MD, USA), and miR-NC or miR-27b mimic. .. Luciferase activity was detected by Tecan Infinite M200 (Tecan, Männedorf, Switzerland) and the luminescent intensity of Firefly luciferase activity derived by normalization with Renilla luciferase as an internal control.

    Article Title: MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells.
    Article Snippet: The FOXO1-related fluorescence was firstly evaluated in EC transfected with fluorescent pEGFP rbFOX1 plasmid (63085, Addgene, Watertown, MA, USA) and Dreamfect 1000 reagent (DF41000, OZ Biosciences, Quimigen, Madrid, Spain), to assess the best transfectant experimental conditions. .. Dual-luciferase reporter assay was then performed by Firefly & Renilla luciferase single tube assay kit (30081-T, Biotium, Fremont, CA, USA), following manufacturer’s protocols, on EC co-transfected with customized luciferase plasmid containing the 350–550 region of the 3′UTR target expression clone for human FOXO1 (CS-HmiT127365-MT06-01, GeneCopoeia, Rockville, MD, USA), and miR-NC or miR-27b mimic. .. Luciferase activity was detected by Tecan Infinite M200 (Tecan, Männedorf, Switzerland) and the luminescent intensity of Firefly luciferase activity derived by normalization with Renilla luciferase as an internal control.

    Luciferase:

    Article Title: MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells
    Article Snippet: The FOXO1-related fluorescence was firstly evaluated in EC transfected with fluorescent pEGFP rbFOX1 plasmid (63085, Addgene, Watertown, MA, USA) and Dreamfect 1000 reagent (DF41000, OZ Biosciences, Quimigen, Madrid, Spain), to assess the best transfectant experimental conditions. .. Dual-luciferase reporter assay was then performed by Firefly & Renilla luciferase single tube assay kit (30081-T, Biotium, Fremont, CA, USA), following manufacturer's protocols, on EC co-transfected with customized luciferase plasmid containing the 350–550 region of the 3′UTR target expression clone for human FOXO1 (CS-HmiT127365-MT06-01, GeneCopoeia, Rockville, MD, USA), and miR-NC or miR-27b mimic. .. Luciferase activity was detected by Tecan Infinite M200 (Tecan, Männedorf, Switzerland) and the luminescent intensity of Firefly luciferase activity derived by normalization with Renilla luciferase as an internal control.

    Article Title: MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells.
    Article Snippet: The FOXO1-related fluorescence was firstly evaluated in EC transfected with fluorescent pEGFP rbFOX1 plasmid (63085, Addgene, Watertown, MA, USA) and Dreamfect 1000 reagent (DF41000, OZ Biosciences, Quimigen, Madrid, Spain), to assess the best transfectant experimental conditions. .. Dual-luciferase reporter assay was then performed by Firefly & Renilla luciferase single tube assay kit (30081-T, Biotium, Fremont, CA, USA), following manufacturer’s protocols, on EC co-transfected with customized luciferase plasmid containing the 350–550 region of the 3′UTR target expression clone for human FOXO1 (CS-HmiT127365-MT06-01, GeneCopoeia, Rockville, MD, USA), and miR-NC or miR-27b mimic. .. Luciferase activity was detected by Tecan Infinite M200 (Tecan, Männedorf, Switzerland) and the luminescent intensity of Firefly luciferase activity derived by normalization with Renilla luciferase as an internal control.

    Plasmid Preparation:

    Article Title: MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells
    Article Snippet: The FOXO1-related fluorescence was firstly evaluated in EC transfected with fluorescent pEGFP rbFOX1 plasmid (63085, Addgene, Watertown, MA, USA) and Dreamfect 1000 reagent (DF41000, OZ Biosciences, Quimigen, Madrid, Spain), to assess the best transfectant experimental conditions. .. Dual-luciferase reporter assay was then performed by Firefly & Renilla luciferase single tube assay kit (30081-T, Biotium, Fremont, CA, USA), following manufacturer's protocols, on EC co-transfected with customized luciferase plasmid containing the 350–550 region of the 3′UTR target expression clone for human FOXO1 (CS-HmiT127365-MT06-01, GeneCopoeia, Rockville, MD, USA), and miR-NC or miR-27b mimic. .. Luciferase activity was detected by Tecan Infinite M200 (Tecan, Männedorf, Switzerland) and the luminescent intensity of Firefly luciferase activity derived by normalization with Renilla luciferase as an internal control.

    Article Title: MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells.
    Article Snippet: The FOXO1-related fluorescence was firstly evaluated in EC transfected with fluorescent pEGFP rbFOX1 plasmid (63085, Addgene, Watertown, MA, USA) and Dreamfect 1000 reagent (DF41000, OZ Biosciences, Quimigen, Madrid, Spain), to assess the best transfectant experimental conditions. .. Dual-luciferase reporter assay was then performed by Firefly & Renilla luciferase single tube assay kit (30081-T, Biotium, Fremont, CA, USA), following manufacturer’s protocols, on EC co-transfected with customized luciferase plasmid containing the 350–550 region of the 3′UTR target expression clone for human FOXO1 (CS-HmiT127365-MT06-01, GeneCopoeia, Rockville, MD, USA), and miR-NC or miR-27b mimic. .. Luciferase activity was detected by Tecan Infinite M200 (Tecan, Männedorf, Switzerland) and the luminescent intensity of Firefly luciferase activity derived by normalization with Renilla luciferase as an internal control.

    Expressing:

    Article Title: MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells
    Article Snippet: The FOXO1-related fluorescence was firstly evaluated in EC transfected with fluorescent pEGFP rbFOX1 plasmid (63085, Addgene, Watertown, MA, USA) and Dreamfect 1000 reagent (DF41000, OZ Biosciences, Quimigen, Madrid, Spain), to assess the best transfectant experimental conditions. .. Dual-luciferase reporter assay was then performed by Firefly & Renilla luciferase single tube assay kit (30081-T, Biotium, Fremont, CA, USA), following manufacturer's protocols, on EC co-transfected with customized luciferase plasmid containing the 350–550 region of the 3′UTR target expression clone for human FOXO1 (CS-HmiT127365-MT06-01, GeneCopoeia, Rockville, MD, USA), and miR-NC or miR-27b mimic. .. Luciferase activity was detected by Tecan Infinite M200 (Tecan, Männedorf, Switzerland) and the luminescent intensity of Firefly luciferase activity derived by normalization with Renilla luciferase as an internal control.

    Article Title: MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells.
    Article Snippet: The FOXO1-related fluorescence was firstly evaluated in EC transfected with fluorescent pEGFP rbFOX1 plasmid (63085, Addgene, Watertown, MA, USA) and Dreamfect 1000 reagent (DF41000, OZ Biosciences, Quimigen, Madrid, Spain), to assess the best transfectant experimental conditions. .. Dual-luciferase reporter assay was then performed by Firefly & Renilla luciferase single tube assay kit (30081-T, Biotium, Fremont, CA, USA), following manufacturer’s protocols, on EC co-transfected with customized luciferase plasmid containing the 350–550 region of the 3′UTR target expression clone for human FOXO1 (CS-HmiT127365-MT06-01, GeneCopoeia, Rockville, MD, USA), and miR-NC or miR-27b mimic. .. Luciferase activity was detected by Tecan Infinite M200 (Tecan, Männedorf, Switzerland) and the luminescent intensity of Firefly luciferase activity derived by normalization with Renilla luciferase as an internal control.



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